Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components?
نویسندگان
چکیده
منابع مشابه
Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components?
In a recently published study, Anna Krichevsky and colleagues raise the important question of whether results of in vitro extracellular RNA (exRNA) studies, including extracellular vesicle (EV) investigations, are confounded by the presence of RNA in cell culture medium components such as foetal bovine serum (FBS). The answer, according to their data, is a resounding "yes". Even after lengthy u...
متن کاملExtracellular Ribonucleic Acids (RNA) Enter the Stage in Cardiovascular Disease.
Inflammatory and ischemic cardiovascular diseases, especially atherosclerosis and myocardial infarction, remain the number one cause of death in the Western world, whereas the therapeutic options currently available are still limited. Several recent findings have indicated that nucleic acids, particularly extracellular ribosomal RNA and micro-RNAs, significantly contribute to the adverse outcom...
متن کاملFetal Bovine Serum RNA Interferes with the Cell Culture derived Extracellular RNA
Fetal bovine serum (FBS) has been used in eukaryotic cell cultures for decades. However, little attention has been paid to the biological effects associated with RNA content of FBS on cell cultures. Here, using RNA sequencing, we demonstrate that FBS contains a diverse repertoire of protein-coding and regulatory RNA species, including mRNA, miRNA, rRNA, and snoRNA. The majority of them (>70%) a...
متن کاملSome clinical trials are driven by fashion, not science.
1 Ioannidis JPA. Clinical trials: what a waste. BMJ 2014;349:g7089. (10 December.) 2 LeFevre ML. Screening for vitamin D deficiency in adults: US Preventive Services Task Force recommendation statement. Ann Intern Med 2014; published online 25 Nov, doi:10. 7326/M14-2450. 3 Fortmann SP, Burda BU, Senger CA, Lin JS, Beil TL, O’Connor E, et al. Vitamin, mineral, and multivitamin supplements for th...
متن کاملOptimization of Culture Conditions and Medium Components for Carboxymethyl Cellulase (CMCase) Production by a Novel Basidiomycete Strain Peniophora sp. NDVN01
Background: Cellulytic enzymes including carboxymethyl cellulases play the key role in hydrolysis of cellulose, a huge organic carbon reservoir on earth, into monomeric sugars and their eventual conversion into valuable chemicals and energy sources. Objectives: In this study, we described the identification of a basidiomycete isolate NDVN01 and optimization of culture conditions and medium com...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Extracellular Vesicles
سال: 2017
ISSN: 2001-3078
DOI: 10.1080/20013078.2016.1272832